Aim: The study was conducted to determine seroprevalence of the five
diseases influenza, Newcastle, Mycoplasma gallisepticum, Mycoplasma synoviae and
salmonella, among around native hens of Eghlid in Iran, on spring 2011.
Materials and Methods: On the basis of native Hens distribution, this region
divided into four parts of Eghlid, Doskord, Sedeh and Hasan-abad. Fifty
unvaccinated native Hens randomly selected from each part. Blood samples were
aseptically collected from the wing veins using 5-ml sterile syringe. Serum from
hens was tested for detection and titration for Mycoplasma and Salmonella by the
rapid slide agglutination method, and was tested for influenza and Newcastle by
the Hemagglutination Inhibition Assay. The data was analyzed completely in
randomized design with four treatments, 50 repetitions for each disease.
Results: 34 out of 200 samples (17%) were positive for influenza. There were
significant differences between regions (p <0.01). 38 out of 200 samples (19%)
were positive for Newcastle. The maximum infectious rate obtained from Eghlid.
There were significant differences between regions (p <0.05). 170 out of 200
samples (85%) were positive for Mycoplasma gallisepticum. 4 from 200 samples
(2%) were positive for Mycoplasma synoviae. The results do not show a
significant difference for salmonella (p <0.05).
Conclusion:
Contamination of Influenza, Newcastle and Mycoplasma gallisepticum was high, and
the highest contamination rate was related to Mycoplasma gallisepticum. It is
usually recommended that preventive strategies, such as appropriate husbandry
and hygiene, sanitary handling of chicks and eggs, routine health monitoring and
vaccination of Native hens should be emphasized.
Keywords: influenza,
mycoplasma, newcastle, salmonella, seroprevalence
